Metabolic Fate of the Increased Yeast Amino Acid Uptake Subsequent to Catabolite Derepression
Direct fluorescence was measured in yeast strains with GFP-tagged CYT1 and CIT1 using Zeiss LSM510 and Bio-Rad Radiance 2100 confocal systems, respectively. (a) Cyt1-GFP fluorescence in yeast grown in 2% glucose YNB compared to (c) in 2% galactose YNB medium. Micrographs (b) and (d) are corresponding Nomarski differential interference contrast images of (a) and (c), respectively. (e) Cit1-GFP fluorescence of yeast grown in 2% glucose YNB compared to (g) grown in 2% galactose YNB medium. (f) and (h) are corresponding bright-field images of (e) and (g), respectively. Representative pictures of experiments in triplicate are presented.
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