DTNQ-Pro, a Mimetic Dipeptide, Sensitizes Human Colon Cancer Cells to 5-Fluorouracil Treatment
Figure 3
Effect of 5-FU D-Caco-2 treatment on TBARS. The cells were seeded in six multiwell plates at the density of 25 × 104 cells/plate and were incubated for the first 48 h with 500 nM DTNQ-Pro (D-Caco-2) the cells were washed to remove unattached dead cells and were incubated with and without 500 nM of 5-FU for subsequently 48 h. (a) TBARS levels in Caco-2 (CTR) and D-Caco-2 cells after 48 h of incubation without and with 500 nM 5-FU; (b) catalase activity. Catalase activity in Caco-2 (CTR) and D-Caco-2 cells after 48 h of incubation without and with 500 nM 5-FU. The bars represent means ± SEM of three independent experiments. Asterisks indicate significant difference between the D-Caco-2-treated samples compared with control value ; ; n.s.: not significant.
