Ischemia Induces Release of Endogenous Amino Acids from the Cerebral Cortex and Cerebellum of Developing and Adult Mice
Table 1
Amino acid contents in slices.
Amino acid (mmol/kg)
Cerebral cortex
Cerebellum
7-day olds
3-month olds
7-day olds
3-month olds
Taurine
296.19 ± 23.41
26.81 ± 2.75*
194.37 ± 12.71
13.17 ± 3.06*
GABA
16.38 ± 1.13
18.41 ± 1.36
13.25 ± 1.80
16.03 ± 1.88
Glycine
24.17 ± 7.42
3.82 ± 0.27*
18.23 ± 1.49
8.52 ± 1.08*
Aspartate
57.82 ± 4.25
20.92 ± 1.54*
50.26 ± 2.64
17.78 ± 1.90*
Glutamate
75.13 ± 5.71
64.99 ± 3.38
54.82 ± 3.18
53.70 ± 6.40
Glutamine
7.54 ± 0.68
6.53 ± 1.03
9.58 ± 1.60
6.26 ± 1.27
Alanine
10.34 ± 3.38
3.62 ± 0.29*
8.50 ± 0.38
4.99 ± 0.74*
Serine
21.33 ± 9.59
5.58 ± 0.40*
9.93 ± 0.38
9.55 ± 1.62
Threonine
5.93 ± 2.06
2.13 ± 0.16*
5.31 ± 0.20
3.11 ± 0.84*
Amino acid concentrations (mean values ± SEM) are given in mmol/kg protein in preincubated slices; that is, they show the amino acid contents before the onset of superfusion. Number of independent experiments 8 (cerebral cortex) and 16 (cerebellum). Significance of differences between 7-day-old and 3-month-old mice: *P < 0.01.
