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Figure 3: Effects of feeding diets differing in sulfur amino acid levels on the abundance of 4EBP1 and eIF4E protein and mRNA in liver of rats. Values are means ± SEM for 4 rats. Values represented by bars not labeled with the same letter are significantly different at by ANOVA and Tukey’s comparison. Representative western blots are shown; these were run by loading equal amounts of total soluble protein per lane with the order of samples being the same as for the bar graphs. Protein values were normalized by β-actin, whereas mRNA abundances were normalized to tubulin.