Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP

<table>Anion-exchange chromatography on DEAE-Sephadex A-50 of SPS E197D preparation. <svg height="11.2625" id="M17" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 14.1625 11.2625" width="14.1625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.2)"><path d="M799 12l-7 -12h-758l-7 12l381 643h10zM711 50l-298 503l-298 -503h596z" id="x25B3"></path></g>
</svg>—the base of the triangle shows the active fractions of SPS, able to bind TNP-ATP.</table>

Journal of Amino Acids

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Figure 4

Figure 4: Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP 

Figure 4 | Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP 