Table of Contents
Journal of Amino Acids
Volume 2014 (2014), Article ID 283962, 9 pages
http://dx.doi.org/10.1155/2014/283962
Research Article

L-Phenylalanine Transport in Saccharomyces cerevisiae: Participation of GAP1, BAP2, and AGP1

Departamento de Bioquímica Humana, Facultad de Medicina, Paraguay 2155, 1121 Buenos Aires, Argentina

Received 4 October 2013; Revised 1 December 2013; Accepted 30 December 2013; Published 20 February 2014

Academic Editor: Dorothy Gietzen

Copyright © 2014 Daniel A. Sáenz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We focused on the participation of GAP1, BAP2, and AGP1 in L-phenylalanine transport in yeast. In order to study the physiological functions of GAP1, BAP2, and AGP1 in L-phenylalanine transport, we examined the kinetics, substrate specificity, and regulation of these systems, employing isogenic haploid strains with the respective genes disrupted individually and in combination. During the characterization of phenylalanine transport, we noted important regulatory phenomena associated with these systems. Our results show that Agp1p is the major transporter of the phenylalanine in a gap1 strain growing in synthetic media with leucine present as an inducer. In a wild type strain grown in the presence of leucine, when ammonium ion was the nitrogen source, Bap2p is the principal phenylalanine carrier.