Properties of Taurine Release in Glucose-Free Media in Hippocampal Slices from Developing and Adult Mice

Table 1

Effects of ion channel inhibitors on taurine release from hippocampal slices from 3-month-old and 7-day-old mice in hypoglycemia.

Effectors	Efflux rate constants (×10^−3 min^−1) ± SEM
	3-month-old				7-day-old
	Basal (control)	2.36 ± 0.11	(20)	1.93 ± 0.18	(11)	1.34 ± 0.08	(31)	1.20 ± 0.11	(11)
+50 mM K^+ (control)			2.92 ± 0.21	(10)			1.90 ± 0.10	(12)
DIDS 0.5 mM	1.34 ± 0.15	(6)	1.31 ± 0.10	(4)	1.12 ± 0.14	(8)	1.16 ± 0.13	(4)
+50 mM K^+			1.81 ± 0.12	(4)			1.45 ± 0.06	(4)
SITS 2.0 mM	1.04 ± 0.07	(8)	0.93 ± 0.06	(4)	0.80 ± 0.09	(6)	0.62 ± 0.06	(4)
+50 mM K^+			1.50 ± 0.21	(4)			0.90 ± 0.12	(4)
9-AC 0.2 mM	2.67 ± 0.17	(5)	2.74 ± 0.49	(4)	1.82 ± 0.23	(8)	1.07 ± 0.09	(4)
+50 mM K^+			3.53 ± 0.32	(4)			1.84 ± 0.10	(4)

The drugs were added at the beginning of superfusion and 50 mM K⁺ at 30 min. The results show the efflux rate constants ± SEM (×10⁻³ min⁻¹) for the time intervals of 20–30 min () and 34–50 min without the excess of K⁺ or in the presence of 50 mM K⁺ () with the number of independent experiments in parenthesis. Abbreviations: DIDS, diisothiocyanostilbene-2′2-disulphonate; SITS, 4-acetamido-4′-isothiocyanostilbene-2′2-disulphonate; 9-AC, 9-anthracenecarboxylic acid. Significance of differences from the corresponding controls: , .