Properties of Taurine Release in Glucose-Free Media in Hippocampal Slices from Developing and Adult Mice

Table 3

Effects of adenosine agonists and riluzole on taurine release from hippocampal slices from 3-month-old and 7-day-old mice in hypoglycemia.

Effectors	Efflux rate constants (×10^−3 min^−1) ± SEM
	3-month-old				7-day-old
	Basal (control)	2.36 ± 0.11	(20)	1.93 ± 0.18	(11)	1.34 ± 0.08	(31)	1.20 ± 0.11	(11)
+50 mM K^+ (control)			2.92 ± 0.21	(10)			1.90 ± 0.10	(12)
CHA 0.5 mM	2.98 ± 0.12	(6)	1.85 ± 0.30	(6)	1.48 ± 0.07	(10)	1.11 ± 0.06	(7)
+50 mM K^+			2.42 ± 0.27	(4)			2.00 ± 0.06	(4)
R-PIA 0.1 mM	2.46 ± 0.17	(6)	1.92 ± 0.21	(6)	1.97 ± 0.19	(6)	1.41 ± 0.12	(4)
+50 mM K^+			3.63 ± 0.51	(4)			2.12 ± 0.11	(4)
CGS 21680 10.0 mM	2.59 ± 0.22	(4)	2.20 ± 0.20	(4)	1.75 ± 0.16	(6)	1.38 ± 0.20	(4)
+50 mM K^+			3.44 ± 0.32	(4)			2.28 ± 0.11	(4)
Riluzole 0.1 mM	2.47 ± 0.17	(7)	1.86 ± 0.27	(4)	1.86 ± 0.15	(6)	1.38 ± 0.10	(4)
+50 mM K^+			2.50 ± 0.35	(4)			2.23 ± 0.15	(4)

The agonists were added at the beginning of superfusion and 50 mM K⁺ at 30 min. The results show the efflux rate constants ± SEM (×10⁻³ min⁻¹) for the time intervals of 20–30 min () and 34–50 min without the excess of K⁺ or in the presence of 50 mM K⁺ () with the number of independent experiments in parenthesis. CHA: N⁶-cyclohexyladenosine; R-PIA: (R(−)N⁶-(2-phenylisopropyl)adenosine; CGS 21680: 4-[2-[[6-amino-9-(N-ethyl-β-D-ribofuranuronamidosyl)-9H-purin-2-yl]amino]ethyl]benzenepropanoic acid hydrochloride. Significant difference from the control: .