Urea Unfolding Study of E. coli Alanyl-tRNA Synthetase and Its Monomeric Variants Proves the Role of C-Terminal Domain in Stability

Figure 3

Change in normalized ANS fluorescence intensity with increased urea concentration. ANS and alaRS concentrations were 30 μM and 4 μM, respectively. Excitation wavelength was 420 nm and the bandpasses for both the excitation and emission were kept at 5 nm. The spectra were measured at 25°C. The samples were kept at dark for 1 h after the ANS addition. The values at 482 nm were plotted after they were normalized considering the 0 M values as 1.