Table of Contents
Journal of Amino Acids
Volume 2016, Article ID 7374316, 8 pages
Research Article

Protein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids

Division 1.5 Protein Analysis, Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Strasse 11, 12489 Berlin, Germany

Received 15 April 2016; Accepted 28 June 2016

Academic Editor: Hieronim Jakubowski

Copyright © 2016 Almut Hesse and Michael G. Weller. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary material shows an extended time window for chromatograms, details of the data evaluation (peak fitting), the estimation of the limit of detection (LOD) of proteins, immobilization protocols of bovine serum albumin (BSA) on solid supports and the quantification of covalently immobilized protein.

  1. Supplementary Material