Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (Nicotiana sylvestris)
Figure 2
SDS-PAGE of N. sylvestris total protein under reducing (R) and nonreducing (NR) conditions. Size exclusion pools A, B, and C under nonreducing conditions showing relative positions of possible trimeric and dimeric complexes and 7S and 11S monomers. Enriched fractions prepared under reducing conditions showing separation of disulphide-dependent components, lanes 1, 2, 7S, and 11S. All gels 4–12% Bis-Tris. Protein was visualized by CBB staining. Arrowheads indicate the position of band excised for mass spectrometric analysis.
