Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (Nicotiana sylvestris)

Figure 5

ClustalW alignment of sequences from D. carota, S. demissum, C. maxima, and G. max. Amino acids in bold print represent sequences obtained from putative vicilin of N. sylvestris where light gray sections indicate identical amino acids. Asterisks (_*) under sequences indicate identically conserved residues while double dots (:) indicate conserved substitutions, and singe dots (⋅) indicate semiconserved substitutions. Approximate beginning and ending of each cupin domain are indicated by Cu > or <Cu, respectively. Cysteine residues are shown in bold print and marked by S. N-glycosylation sites (Asn-Xaa-Ser/Thr) are marked in gray boxes.