Selection of Housekeeping Genes for Transgene Expression Analysis in Eucommia ulmoides Oliver Using Real-Time RT-PCR

Table 3

ANOVA F-test of candidate housekeeping gene variances among three transgenic types and wild-type as well as among transgenic lines within each transgenic type.

	ACTα	ARPT	CYP	EF1  𝛼	EIF1  𝛼	GAPD	rbcL	TUBα	TUBβ	UBQ
Types	3.99	5.30*	3.87	2.66	11.25**	0.39	10.73**	8.19*	4.57	93.55**
Lines	1.55	8.70**	2.47	2.26	8.06**	5.43**	7.20**	1.63	2.07	0.46

Data are based on variance components and mixed model ANOVA/ANCOVA using relative quantities of each candidate housekeeping gene in all samples; *, 𝑃<.05; **, 𝑃<.01.