Improved and Reproducible Flow Cytometry Methodology for Nuclei Isolation from Single Root Meristem
Figure 3
Morphological analysis of DAPI-stained nuclei, isolated from single onion root meristem after 2- (a), 4- (b) and 6- (c) pulse homogenizations. Note that after running of 6 pulses the nuclei were well preserved, isolated and showing no residual cytoplasm. Bar = 10 m.