On the Tempo of Genome Size Evolution in Angiosperms

Table 1

Gene regions specified in the mega-phylogeny construction of Monocotyledonae (monocots) and Fabaceae (legumes). The median absolute deviation (MAD) was used to assess sequence saturation and to parse sequences into separate files based on NCBI taxonomy and brought together again using NCBI-based guide tree and profile-to-profile alignment methodology (see Methods and Materials).

Phylogeny	Gene region	Description	N	MAD	Profiles
Monocotyledonae	atpB	Atp synthase beta chain	128	0.006	none
Monocotyledonae	ITS	Internal transcribed spacer 1, 5.8S ribosomal RNA, and internal transcribed spacer 2	596	0.108	mostly to tribe and genus
Monocotyledonae	matK	Maturase K	310	0.068	mostly to family
Monocotyledonae	ndhF	NADH-plastoquinone oxidoreductase	203	0.021	mostly to order
Monocotyledonae	rbcL	Ribulose bisphosphate carboxylase	270	0.005	none
Monocotyledonae	rps16	Ribosomal protein S16 intron	88	0.014	mostly to order
Monocotyledonae	trnL-trnF	trnL-trnF intergenic spacer	472	0.033	mostly to family
Fabaceae	ETS	External transcribed spacer and 18S ribosomal RNA	18	0.064	mostly to tribe and genus
Fabaceae	ITS	Internal transcribed spacer 1, 5.8S ribosomal RNA, and internal transcribed spacer 2	293	0.031	mostly to tribe and genus
Fabaceae	matK	Maturase K	119	0.014	mostly to tribe and genus
Fabaceae	psbA-trnH	psbA-trnH intergenic spacer	31	0.005	none
Fabaceae	rbcL	Ribulose bisphosphate carboxylase	66	0.001	none
Fabaceae	trnL-trnF	trnL-trnF intergenic spacer	81	0.040	mostly to tribe

MAD scores in bold italics indicate the gene region was saturated across the most inclusive taxonomic-level and broken up into profiles of various taxonomic levels.
N indicates the number of sequences in GenBank returned according to our input search list; however, due to synonymy and errors in GenBank the final tree was pruned to exactly match our genome size data set.