Flow cytometry (FCM) histograms showing relative nuclear DNA content of Duckweed. (a) Histogram showing relative DNA content of Spirodela polyrhiza (1, 151 Mbp) and internal standard Brachypodium distachyon Bd21 (2, 300 Mbp) based on relative PI fluorescent intensity (channel number). Linear PI fluorescence intensity of G1 nuclei was used for the calculation of DNA content (3500 particles were counted); (b) Difference in relative DNA content of two simultaneously measured Lemna minor accessions (2, Lm6591, 444 Mbp; 3, Lm7436, 560 Mbp) with internal standard Bd21 (1); 5000 particles were counted. (c) Difference in relative DNA content of two simultaneously measured Lemna aequinoctialis accessions (2, La6612, 410 Mbp; 3, La7126, 748 Mbp) with internal standard Bd21 (1); 5000 particles were counted. (d) Summary of Panel a, b, and c and genome size corresponding to each peak.
