Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum: Live Cell Labeling with Monoclonal Antibodies
Figure 1
Live cell labels. (a) represents a cell labeled with the HG-specific mAb, LM18. Note the punctate appearance of the HGs on the wall surface. Scale bar = 10 μm. (b) is a magnified view of the HG lattice, labeled with JIM5, on the outer portion of the cell. Scale bar = 4.75 μm. (c) represents a cell first labeled with JIM5, recultured for 24 h, and then relabeled with JIM7. Scale bar = 14.8 μm. (d) represents a cell labeled with JIM5 and recultured for 24 h. The dark zone in the center represents where HGs are secreted. (e) is a cell labeled with the AGP-binding mAb, JIM13. A finely labeled sheath made of numerous puncta is labeled on the outer wall surface (arrows). Scale bar = 14.8 μm. (f) is cell labeled with an anti-EPS antibody and highlights the extensive sheath that surrounds the cell (arrows). Scale bar = 14.8 μm.
