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Journal of Botany
Volume 2012 (2012), Article ID 137037, 6 pages
http://dx.doi.org/10.1155/2012/137037
Research Article

The Role of Pathogenesis-Related Proteins in the Tomato-Rhizoctonia solani Interaction

Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 1163, Mashhad 9177948978, Iran

Received 19 September 2011; Accepted 19 December 2011

Academic Editor: Olivier Honnay

Copyright © 2012 Parissa Taheri and Saeed Tarighi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rhizoctonia solani is one of the most destructive pathogens causing foot rot disease on tomato. In this study, the molecular and cellular changes of a partially resistant (Sunny 6066) and a susceptible (Rio Grande) tomato cultivar after infection with necrotrophic soil-borne fungus R. solani were compared. The expression of defense-related genes such as chitinase (LOC544149) and peroxidase (CEVI-1) in infected tomato cultivars was investigated using semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). This method revealed elevated levels of expression for both genes in the partially resistant cultivar compared to the susceptible cultivar. One of the most prominent facets of basal plant defense responses is the formation of physical barriers at sites of attempted fungal penetration. These structures are produced around the sites of potential pathogen ingress to prevent pathogen progress in plant tissues. We investigated formation of lignin, as one of the most important structural barriers affecting plant resistance, using thioglycolic acid assay. A correlation was found between lignification and higher level of resistance in Sunny 6066 compared to Rio Grande cultivar. These findings suggest the involvement of chitinase, peroxidase, and lignin formation in defense responses of tomato plants against R. solani as a destructive pathogen.