Genome Mutation Revealed by Artificial Hybridization between Chrysanthemum yoshinaganthum and Chrysanthemum vestitum Assessed by FISH and GISH
Figure 2
Tricolor FISH ((a), (c), (e), and (f).) and bicolor ((b) and (d)) applied on mitotic chromosomes in the artificial interspecific F1 hybrid between Ch. yoshinaganthum and Ch. vestitum (, pentaploid, ID: y-11-4-1-10 ((b) and (d)), y-11-4-1-2 ((c) and (f) I), and y-11-4-1-5 ((e) and (f) II). (a), (c), (e), and (f). Superimposed image locates the distribution of the yellow signals of seven yellow-green-color signals ((c) and (f) I) of 5S rDNA sites and 14 ((e) and (f) II) on seven and thirteen chromosomes and ten red signals ((c) and (f) I) and 11 ((e) and (f) II) of 45S rDNA sites in ten and eleven chromosomes that were detected by the probes. (b) and (d). Yellow-color signals of the telomeres that were detected by the biotin-labeled telomeric probe in interphase (b) and terminal sites in metaphase (d). All chromosomes showed terminal signals except four chromosomes that showed subterminal sites of telomeres (pointed by four arrows). Bar = 10 μm.
