Review of the Genus Andropogon (Poaceae: Andropogoneae) in America Based on Cytogenetic Studies
Figure 1
GISH on mitotic and meiotic metaphase chromosomes of (a–d) diploid () and (e–h) hexaploid () Andropogon species. (a) and (b) Mitotic chromosomes of SA diploid A. selloanus probed with genomic DNA from SA diploid A. macrothrix and detected with green fluorescence. (a) DAPI-stained chromosomes; (b) GISH showing all 20 chromosomes fluorescing green. (c) and (d) Meiotic chromosomes of SA diploid A. selloanus probed with genomic DNA from the NA diploid A. gyrans and detected with green fluorescence. (c) DAPI-stained chromosomes; (d) GISH showing all 10 bivalents fluorescing green. (e) and (f) Mitotic chromosomes of SA hexaploid A. lateralis probed simultaneously with genomic DNA from the SA diploid A. selloanus (green fluorescence) and pTa71 (red fluorescence). (e) DAPI-stained chromosomes; (f) GISH showing 20 chromosomes fluorescing green (S genome chromosomes, arrows) and two sites of red hybridization (asterisks) corresponding to the location of the 18S.25S rDNA on two chromosomes that do not fluoresce green and thus do not originate from the S genome. (g) and (h) Meiotic chromosomes of SA hexaploid A. lateralis probed with genomic DNA from the NA diploid A. gyrans and detected with red fluorescence. (g) DAPI-stained chromosomes; (h) GISH showing approximately 10 bivalents fluorescing red. Scale bar = 2 μm. Figure extracted from [29].
