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Journal of Botany
Volume 2013, Article ID 819531, 9 pages
http://dx.doi.org/10.1155/2013/819531
Research Article

Indole-3-Acetic Acid, Polyamines, and Phenols in Hardwood Cuttings of Recalcitrant-to-Root Wild Grapes Native to East Asia: Vitis davidii and Vitis kiusiana

1Graduate School of Life & Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan
2Faculty of Agriculture, Kochi University, Otsu 200, Monobe, Nankoku, Kochi 783-8502, Japan

Received 4 July 2013; Revised 20 August 2013; Accepted 7 October 2013

Academic Editor: Bernd Schneider

Copyright © 2013 Shuji Shiozaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Levels of indole-3-acetic acid (IAA), free, conjugated, and bound polyamines (PAs), and phenols were estimated in the basal portion of hardwood cuttings of Vitis davidii and V. kiusiana in relation to rooting and compared with Vitis labruscana Bailey cv. Campbell Early. A high rate of root formation was observed in ‘Campbell Early’ cuttings 60 days after planting. However, none of the V. davidii and V. kiusiana cuttings formed roots. An increase in IAA contents was observed in ‘Campbell Early’ cuttings, but not in V. davidii and V. kiusiana, 60 days after planting. The content of free, conjugated, and bound PAs was either constant or decreased in ‘Campbell Early’ cuttings during planting and was especially decreased in conjugated putrescine and spermidine. An increase in free and conjugated putrescine was observed after planting in V. kiusiana cuttings. In the cuttings of V. davidii, only bound spermine increased after planting. The content of total phenols, orthodiphenols, total catechins, and gallocatechin derivatives decreased after planting in ‘Campbell Early’ cuttings. Orthodiphenols and total catechin contents increased in the cuttings of V. kiusiana, and gallocatechin derivatives increased in V. davidii. The relationships between these endogenous factors and the recalcitrant-to-root features of V. davidii and V. kiusiana cuttings are discussed.