Research Article
Anatomy and Histochemistry of Roots and Shoots in Wild Rice (Zizania latifolia Griseb.)
Figure 1
(a)–(h) Photomicrographs of Zizania latifolia adventitious roots; scale bars = 50 μm. (a) Root 155 mm long, sectioned at 5 mm behind tip; endodermis (arrowheads); adjacent endodermal cortical layers with thickened walls (asterisk); cortex; schizolysigeny aerenchyma; hypodermis; epidermis; sclerenchyma layer; protoxylem; metaxylem; staining: TBO. (b) Root 155 mm long, sectioned at 10 mm; uniseriate exodermis with weakly Casparian bands (arrows); protoxylem; staining: BAB. (c) Root 155 mm long at 30 mm; lignified adjacent endodermal cortical layers (asterisk); uniseriate exodermis with Casparian bands (arrows); uniseriate SC; protoxylem; staining: BAB. (d) Root 155 mm long at 30 mm; endodermis suberin lamellae (arrowheads); adjacent endodermal cortical layers suberin lamellae (asterisk); schizolysigeny aerenchyma; suberized SC and exodermis (arrows); staining: SR7B. (e) Root 155 mm long at 50 mm; endodermis Casparian bands (arrowheads); lignified adjacent endodermal cortical layers (asterisk); uniseriate exodermis Casparian bands (arrows); lignified uniseriate SC; staining: BAB. (f) Root 155 mm long at 50 mm; endodermis suberin lamellae (arrowheads); schizolysigeny aerenchyma; suberized SC and exodermis (arrows); staining: SR7B. (g) Root 155 mm long at 50 mm; lignified endodermis (arrowheads); lignified adjacent endodermal cortical layers (asterisk); lignified SC and exodermis (arrows); schizolysigeny aerenchyma; lignified stele pith cells; metaxylem; staining: Pg; inset show protoxylem elements; staining: berberine. (h) Root 155 mm long at 70 mm; endodermis Casparian bands (arrowheads); lignified adjacent endodermal cortical layers (asterisk); uniseriate exodermis Casparian bands (arrows); lignified uniseriate SC; lignified stele pith cells; staining: BAB. cortex, co; epidermis, ep; hypodermis, hy; metaxylem, me; protoxylem, px; schizolysigenous aerenchyma, ae; sclerenchyma layer, SC; stele, st.
