Research Article
Anatomy and Histochemistry of Roots and Shoots in Wild Rice (Zizania latifolia Griseb.)
Figure 2
(a)–(d) Photomicrographs of Zizania latifolia culm (200–280 mm long, 5–8 nodes) sectioned at the first internode (young) and at 3th internode (aged); (e)-(f) stolons (900 mm long, 8–11 nodes are aged) sectioned at the first internode; (g)-(h) rhizomes (800–910 mm long, 20–24 nodes) sectioned at the 5th internode (aged); (i)-(j) leaf sheath and leaf sectioned; scale bars = 100 μm. (a). Young culm; epidermis outer tangential walls; staining: BAB. (b) Young culm; outer layer of PMR with suberin lamellae and suberized epidermis (bracket); schizolysigenous aerenchyma; staining: SR7B; bar = 50 μm. (c) Aged culm; suberized SCR (brace); suberized PMR (bracket); suberized vascular bundle cells; pith cavity; schizolysigeny aerenchyma; staining: SR7B. (d). Aged culm; lignified vascular bundle cells; lignified PMR (bracket); staining: BAB. (e) Aged stolon; SCR (brace); suberized PMR (bracket); suberized vascular bundles; pith cavity; schizolysigeny aerenchyma; staining: SR7B; inset shows suberized PMR (bracket) with suberin lamellae; bar = 50 um. (f) Aged stolon; SCR (brace); lignified vascular bundle cells; lignified PMR (bracket); pith cavity; schizolysigeny aerenchyma; staining: Pg. (g) Aged rhizome; SCR (brace); suberized PMR (bracket); suberized vascular bundle cells; pith cavity; schizolysigeny aerenchyma; staining: SR7B. (h) Aged rhizome; SCR (brace); lignified vascular bundle cells; lignified PMR (bracket); pith cavity; schizolysigeny aerenchyma; staining: Pg. (i) Leaf sheath; cuticle on adaxial surface (arrowhead) and abaxial surface (arrow) of leaf sheath, suberin lamellae on vascular bundle cells; schizolysigeny aerenchyma; staining: SR7B. (j) Leaf blade; cuticle on adaxial surface (arrowhead) and abaxial surface (arrow) of leaf blade; vascular bundle; aerenchyma; staining: SR7B. epidermal cell wall, ew.
