Anatomy and Histochemistry of Roots and Shoots in Wild Rice (Zizania latifolia Griseb.)
Figure 3
Apoplastic barriers permeability tests on roots and rhizomes collected from grow in wetland with berberine tracer. Roots segments sectioned at 30 mm to 60 mm from the tip of 130 mm long roots and sealed ends. The aged rhizome (as stem representative for culms and stolons have similar structure and histochemistry) samples excised with one internode and two end nodes and not sealed ends; except where noted, scale bars = 100 μm. (a) Roots with aerenchyma; thickened adjacent endodermal cortical layers (asterisk), endodermis (arrowhead) and exodermis (arrow) cell walls faintly brown; unstained; bar = 50 μm. (b) The epidermis and PMR of rhizomes faintly brown (arrow); unstained. (c) Roots with aerenchyma; thickened adjacent endodermal cortical layers (asterisk), endodermis (arrowhead) and exodermis cell walls (arrow) fluoresced yellow; crystals of berberine thiocyanate (cross) enter into exodermis or out of it; berberine stained; bar = 50 μm. (d). The epidermis and PMR of rhizomes fluoresced yellow (arrow); berberine stained. (e) Roots with aerenchyma; thickened adjacent endodermal cortical layers (asterisk), endodermis (arrowhead), and exodermis cell walls (arrow) fluoresced yellow; crystals of berberine thiocyanate (cross) enter into exodermis or out of it; berberine and KSCN stained. (f) The epidermis and PMR of rhizomes fluoresced yellow (arrow); berberine and KSCN stained. (g) Roots with ruptured exodermis; crystals of berberine thiocyanate enter into aerenchyma (arrowhead) and exodermis (arrow); berberine and KSCN stained; bar = 50 μm. (h) The transverse sections of rhizomes internodes; crystals of berberine thiocyanate (arrowhead) adhere to aerenchyma and pith cavity; berberine and KSCN stained.
