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Journal of Botany
Volume 2014 (2014), Article ID 735760, 7 pages
Research Article

Zinc Induced Enzymatic Defense Mechanisms in Rhizoctonia Root Rot Infected Clusterbean Seedlings

1Department of Biochemistry, CCS Haryana Agricultural University, Hisar, Haryana 125004, India
2Department of Pathology, CCS Haryana Agricultural University, Hisar, Haryana 125004, India

Received 25 February 2014; Revised 6 May 2014; Accepted 9 May 2014; Published 26 May 2014

Academic Editor: Urs Feller

Copyright © 2014 Neha Wadhwa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This investigation was planned to determine the effect of different concentrations of zinc (Zn) on biochemical constituents of clusterbean, which play an important role in disease resistance mechanisms. Clusterbean seedlings were grown with 0, 10, or 20 mg Zn kg−1 soil treatments in earthen pots filled with 700 g inoculated soil. Soil was inoculated by pretreatment with 250 mg (wet weight) of Rhizoctonia inoculums per pot. A similar set was maintained in uninoculated soil. Root rot incidence decreased to 41 and 27 per cent with 10 and 20 mg Zn kg−1 soil treatments, respectively, as compared to 68 percent at control. Antioxidative enzyme activity (polyphenol oxidase, peroxidase, phenylalanine ammonia lyase, and tyrosine ammonia lyase) increased in inoculated seedlings and was increased further by 20 mg Zn kg−1 soil treatment. Antioxidative enzymes play an important role against fungal invasion, as peroxidase is involved in the formation of barrier via lignifications at the site of pathogen penetration. PAL and TAL play a key role in phenylpropanoid metabolism and could perform defense-related functions. Zn acts as a cofactor for these enzymes, so it can be concluded that Zn may be used as a soil-nutritive agent to increase resistance in plants against fungal diseases.