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Journal of Botany
Volume 2014 (2014), Article ID 949028, 8 pages
Research Article

In Vitro Conservation of Some Threatened and Economically Important Ferns Belonging to the Indian Subcontinent

1Plant Tissue Culture Laboratory, Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CIMAP), Council of Scientific and Industrial Research, P.O. CIMAP, Lucknow 226015, India
2Pteridology Laboratory, National Botanical Research Institute (NBRI), Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, India

Received 1 May 2014; Revised 17 June 2014; Accepted 26 June 2014; Published 10 July 2014

Academic Editor: Curtis C. Daehler

Copyright © 2014 Shastri P. Shukla and P. B. Khare. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was undertaken to identify methods of mass multiplication for five ornamental, economically important ferns (Nephrolepis biserrata (Sw.) Schott., N. cordifolia cv. ‘‘duffii’’ (L.) Presl., N. exaltata cv. bostoniensis (L.) Schott., Pteris vittata L., and Cyclosorus dentatus Link.,) and three threatened ferns, namely, Cyathea spinulosa Wall. ex. Hook, Pityrogramma calomelanos (L.) Link., and Microsorum punctatum (L.) Schott., through in vitro techniques. Collections were made from different biodiversity zones of India including Northeast Himalayas, Kumaon Himalayas, and Western Ghat and successfully introduced and grown in a fern-house. Aseptic cultures were raised at the morphogenic level of callus, axillary shoot, multiple shoot, and rooted plants. An optimized medium is described for each fern species. Plantlets were also produced from spore culture of Cyathea spinulosa and successfully hardened under fern house conditions.