In Vitro Conservation of Some Threatened and Economically Important Ferns Belonging to the Indian Subcontinent

Table 2

Morphogenic level along with the optimised medium and glasshouse hardening status of 8 plant species processed for in vitro conservation.

S. No	Plant species	Explant used	Callus (GR in mgL^−1)	Cultures established at the level of axillary/multiple shoots (GR in mgL^−1)	Rooted plants (GR in mgL^−1)	Number of replicates	Time taken/success rate during hardening
1	Nephrolepis biserrata	Stolon	—	P&T + 2,4-D (2.0) + NAA (2.0)	P&T + IAA (1.0)	10	2 wks/100%
2	N. cordifolia cv. duffii	Leaf primordium	—	P&T + 2,4-D (2.0) + BAP (2.0)	P&T + NAA (2.0); + Kn (1.0)	10	3-4 wks/60%
3	N. exaltata cv. bostoniensis	Leaf primordium	—	MS + 2,4-D (1.0) + BAP (0.5)	—	10	—
4	Cyathea spinulosa	Leaf primordium procured from in vitro-raised sporophytes	KnD + 2,4-D (2.0)	P&T + BAP (2.0) + NAA (1.0)	P&T + IBA (1.0)	10	3-4 wks/100%
		Spore	—	P&T basal media	P&T basal media	10	3-4 wks/80%
5	Pityrogramma calomelanos	Circinate part of young fronds	KnD + 2,4-D (1.0)	—	—	10	—
		Spore	—	P&T basal media	P&T basal media	10	5–7 wks/70%
6	Microsorum punctatum	Leaf primordium	1/4 strength of MS medium (Hormone- free)	—	—	10	—
		Spore	—	P&T basal media	P&T basal media	10	5–7 wks/50%
7	Pteris vittata	Leaf primordium	MS + 2,4-D; (2.0) + BAP; (0.5); GGBs-; MS + 2,4-D; (0.5) + BAP (0.5)	MS + IAA (0.5); + BAP (2.0)	P&T + NAA (1.0); + BAP (0.5)	10	3-4 wks/100%
8	Cyclosorus dentatus	Leaf primordium	—	P&T + BAP (1.0) + IAA (0.5)		10