The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

Figure 2

Regeneration of plant from Ri-transformed roots of T. indica via spontaneous somatic embryogenesis on solid MS medium under photoperiod of 16/8 hours. (a) Spontaneous induction of callus in the older region of transformed roots after 8 weeks of subculture (bar = 0.33 cm), (b) spontaneously developing somatic embryos from the embryogenic calli (bar = 1.1 cm), (c) single somatic embryo transferred to solid MS medium (bar = 0.1 cm), (d) germinated somatic embryo developing into plantlet after 14 days (bar = 0.14 cm), (e) Ri-transformed plantlet developed from somatic embryo after 28 days (bar = 0.25 cm), (f) SE showing stunted development of shoot pole only (bar = 0.12 cm), and (g) undeveloped SE with abnormal shoot bud and root which turned brown (bar = 0.2 cm).

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