The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

Figure 6

Agarose (1.2% (w/v)) gel electrophoresis of (a) PCR products of Ri-transformed plants with rolA-, rolB-, rolC-, and rolD-specific primers, (b) expression of rol genes at the transcriptional level in Ri-transformed plants observed by RT-PCR using rolA-, rolB-, rolC-, and rolD-specific primers. Lane 1: molecular markers (100 bp plus DNA ladder); Lane 2: positive control (pLJ1, containing TL-DNA); Lane 3: negative control (genomic DNA of nontransformed plant); Lanes 4–19: Ri-transformed plant lines; Lanes 4–7: Ri-transformed plant line A₄28#2; Lanes 8–11: Ri-transformed plant line A₄28#1-V; Lanes 12–15: Ri-transformed plant line A₄22#2; Lanes 16–19: Ri-transformed plant line A₄22#1.

(a) PCR

(b) RT-PCR