The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

Table 4

Evaluation of tylophorine content in Ri-transformed plant lines after 12 and 36 months of culture period.

Ri-transformed plant line	Tylophorine content mg gDW^−1
	After 12 months	After 36 months
NT	1.24 ± 0.05	1.21 ± 0.03
A422#6	2.21 ± 0.03	2.23 ± 0.02
A422#7	2.50 ± 0.05	2.52 ± 0.03
A422#8	2.16 ± 0.02	2.13 ± 0.02
A422#9	2.00 ± 0.06	2.05 ± 0.05
A422#10	1.70 ± 0.02	1.72 ± 0.02
A428#6	1.83 ± 0.03	1.81 ± 0.05
A428#7	2.07 ± 0.05	2.09 ± 0.03
A428#8	2.32 ± 0.02	2.30 ± 0.04
A428#9	1.97 ± 0.03	2.00 ± 0.05
A428#10	2.15 ± 0.03	2.14 ± 0.02
A428#1-V	2.93 ± 0.03	2.87 ± 0.02

Values represent mean ± SD. Means with same letter in a column or with prime in a row for each Ri-transformed plant line were not significantly different at according to ANOVA and Tukey’s multiple comparison test.