Table of Contents
Journal of Botany
Volume 2015, Article ID 824683, 8 pages
http://dx.doi.org/10.1155/2015/824683
Research Article

Inhibitory Effect of Heracleum persicum and Ziziphus jujuba on Activity of Alpha-Amylase

1Department of Clinical Biochemistry, Faculty of Medicine, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 15794-61357, Iran
2Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 15794-61357, Iran
3Department of Pharmacognosy, Faculty of Pharmacy, Herbal Medicine & Natural Product Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 15794-61357, Iran

Received 20 July 2015; Revised 14 September 2015; Accepted 21 September 2015

Academic Editor: Hiroshi Tobe

Copyright © 2015 Reza Afrisham et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Postprandial hyperglycemia plays an important role in the development of type 2 diabetes. Inhibition of alpha-amylase was led to a delay in breaks down of starch and glycogen and prevented a rapid rise in blood sugar. Alpha-amylase was isolated by gel filtration chromatography Sephadex G-75 from bovine pancreas. Then, total methanolic extracts of plants were prepared and IC50 values of extracts on alpha-amylase were obtained and compared with acarbose IC50. The polyphenolic content of extracts and antioxidant capacity were determined by Folin-Ciocalteu test and DPPH test, respectively. The specific activity of alpha-amylase was 48.2 U/mg. For inhibition of alpha-amylase, IC50 values of H. persicum, Z. jujuba, and acarbose were 307, 827, and 113 μg/ml, respectively. For inhibition of DPPH radical, IC50 values of extracts were 235 and 701 μg/ml. Total phenolic contents of methanol extracts were and μg tannic acid equivalent/mg extract. Acarbose causes gastrointestinal symptoms and liver toxicity, but H. persicum and Z. jujuba decrease these side effects and prevent gastrointestinal disorders. Due to the high polyphenolic content and antioxidant capacity of these plants and significant inhibitory effect of the plants on alpha-amylase, these plants can be proposed for treatment of diabetic patients.