Table 1: Mobile phase, spray reagent used, and the changed colour for various secondary metabolites by using HPTLC for methanolic leaf and root extracts of Hypochaeris radicata.

Name of the compoundMobile phaseSpray reagentColour of the spot/band
Visible lightUV 366 nm

AlkaloidsEthyl acetate-methanol-water
(10 : 1.35 : 1)
Dragendorff’s reagent followed by 10% ethanolic sulphuric acid reagentYellow, orange-yellow

FlavonoidsToluene-acetone-formic acid
(4.5 : 4.5 : 1)
1% ethanolic Aluminium chloride reagentYellow, yellowish blue

GlycosidesEthyl acetate-ethanol-water
(8 : 2 : 1.2)
Anisaldehyde sulphuric acid reagentPinkish violet

SaponinsChloroform-glacial acetic acid-methanol-Water
(6.4 : 3.2 : 1.2 : 0.8)
Anisaldehyde sulphuric acid reagentBlue, yellow, green, and violet

Terpenoidsn-Hexane-ethyl acetate
(7.2 : 2.9)
Anisaldehyde sulphuric acid reagentBlue, bluish violet