HPTLC Fingerprints of Various Secondary Metabolites in the Traditional Medicinal Herb Hypochaeris radicata L.

Table 1

Mobile phase, spray reagent used, and the changed colour for various secondary metabolites by using HPTLC for methanolic leaf and root extracts of Hypochaeris radicata.

Name of the compound	Mobile phase	Spray reagent	Colour of the spot/band
			Visible light	UV 366 nm
Alkaloids	Ethyl acetate-methanol-water; (10 : 1.35 : 1)	Dragendorff’s reagent followed by 10% ethanolic sulphuric acid reagent	Yellow, orange-yellow	—
Flavonoids	Toluene-acetone-formic acid; (4.5 : 4.5 : 1)	1% ethanolic Aluminium chloride reagent	—	Yellow, yellowish blue
Glycosides	Ethyl acetate-ethanol-water; (8 : 2 : 1.2)	Anisaldehyde sulphuric acid reagent	Pinkish violet	—
Saponins	Chloroform-glacial acetic acid-methanol-Water; (6.4 : 3.2 : 1.2 : 0.8)	Anisaldehyde sulphuric acid reagent	Blue, yellow, green, and violet	—
Terpenoids	n-Hexane-ethyl acetate; (7.2 : 2.9)	Anisaldehyde sulphuric acid reagent	Blue, bluish violet	—