A Panel of Cancer Testis Antigens and Clinical Risk Factors to Predict Metastasis in Colorectal Cancer

Table 1

Primers and condition of RT-PCR analysis.

Genes	Primers sequence (5′→3′)	Conditions
		Denaturation	Annealing	Extension	Cycle (no.)
PAGE4	GATGTGGTTGTATTCGTG; ATCTCCTGCTTCTTTAGTC	94°C—1 min	57°C—30 s	72°C—1 min	35
SCP-1	CCAAAGGCATATACAGTGAAGA; CAGGGGTTGTTAGAGATGAAGG	94°C—1 min	62°C—45 s	72°C—1 min	35
SPANXA/D	GACAAACAATCCAGTGCC; TCCTCCTGTAGCGAACCA	94°C—1 min	57°C—1 min	72°C—1 min	35