Table of Contents
Journal of Biomarkers
Volume 2016 (2016), Article ID 1274603, 11 pages
http://dx.doi.org/10.1155/2016/1274603
Research Article

A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts

1Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA
2Cancer Pharmacology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA
3Early Discovery, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA
4Protein Sciences, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA

Received 1 March 2016; Accepted 21 April 2016

Academic Editor: Yuen-Li Chung

Copyright © 2016 Helen He et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue.