Antidepressant Interactions with the NMDA NR1-1b Subunit

Figure 5

Protease protection assay of NR1-1b S1S2:TCA complexes. To 5.29𝜇M NR1-1b S1S2 in buffer 3 was added the final quenching concentrations of TCAs prior to addition of a 1 : 1 molar ratio of trypsin. The results of the trypsin cuts were visualized by 12% SDS PAGE. Lanes were loaded as follows, (1): desipramine-bound, (2): imipramine-bound, (3): apo NR1-1b S1S2, (4): nortriptyline-bound, (5): maprotiline-bound, (6): trimipramine-bound, (7): trypsin, (8): uncut NR1-1b S1S2.