Journal of Biophysics / 2009 / Article / Tab 2

Research Article

Tropomyosin Period 3 Is Essential for Enhancement of Isometric Tension in Thin Filament-Reconstituted Bovine Myocardium

Table 2

Ca2+ sensitivity and cooperativity of activation. *P value ≤.05 compared to nfTm; **P value ≤.01 compared to nfTm; else, P value >.05 (no significant difference from nfTm). The Hill factor and pCa50 (Ca sensitivity) were deduced by fitting the pCa-tension (or pCa-stiffness) data to (1). In all experiments, temperature was 25°C, ionic strength was 200 mM, and pH was adjusted to 7.00. The data were individually fitted to (1), averaged, and tabulated with ±SEM. Data from [13].

nfTm (control) Acetyl TmUnits

Number of expts.1415159
Based on tension
h1.73 ± 0.211.79 ± 0.191.35 ± 0.221.74 ± 0.23
pC505.28 ± 0.045.11 ± 0.07*5.51 ± 0.08**5.40 ± 0.08log10
Based on stiffness
h1.78 ± 0.111.86 ± 0.161.56 ± 0.221.97 ± 0.37
pCa505.33±0.035.23±0.05*5.52±0.07**5.41±0.09log10

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