Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3

Table 4

Changes in denaturation temperatures for Pf dpd and Zn-Pfdpd in the presence of metal ions at pH 7.8.

(mM)	Phdpd		Zn-Phdpd
	𝑇𝑚(^∘C)	Δ𝑇𝑚	𝑇𝑚(^∘C)	Δ𝑇𝑚
Zn
0.05			118.4	16.9
0.02	111.6	7.2	118.4	16.9
0.01	110.9	6.5	118.4	16.9
Co
0.05	110.7	6.3	116.5	15.0
0.02	110.3	5.9	114.7	13.2
0.01	109.9	5.5	112.9	11.4
Mn
0.05	110.1	5.7	114.5	13.0
0.02	109.7	5.3	112.2	10.7
0.01	109	4.6	109.8	8.3

The denaturation temperatures (𝑇_𝑚) were obtained from the peak temperatures of DSC curves. The 𝑇_𝑚 values of Pf dpd and Zn-Pf dpd in the absence of metals were 104.4 and 101.5^∘C, respectively. All protein concentrations were 0.01 mM.