Exploring the Membrane Mechanism of the Bioactive Peptaibol Ampullosporin A Using Lipid Monolayers and Supported Biomimetic Membranes
Figure 5
Lipid mobility changes associated with AmpA interaction with membranes. (a) Overlay plots of the modification of the lipid diffusion coefficient as a function of AmpA concentration in the SLB, the HBM, and the -BLM. Measurements were performed within an AmpA concentration range from 0 to 10 μM with the HBM, and the -BLM and from 0 to 3 μM with the SLB. (b) Fluorescence emission intensity of NBD-DPPE incorporated in the lipid layers as a function of AmpA concentration at after peptide injection. The fluorescence intensity was normalized at 100% for all the lipid layers before peptide injections.
