Quality of Red Blood Cells Isolated from Umbilical Cord Blood Stored at Room Temperature
Table 2
Comparison of cord blood characteristics and cord RBC quality measures between cord blood groups based on baby’s gender and a method of cord blood collection.
Cord blood characteristics and cord RBC quality parameters
Baby’s gender
Method of cord blood collection
Male ()
Female ()
value
Delivered placenta ()
Placenta in utero ()
value
Age of cord blood
30.2 (11.8)
31.2 (8.3)
0.7908
24.5 (0.4)
31.3 (10.6)
0.0028*
Cord blood volume
60 (23)
70 (34)
0.3310
61 (25)
65 (29)
0.8113
Cord blood hematocrit
0.30 (0.05)
0.31 (0.04)
0.7943
0.31 (0.03)
0.30 (0.05)
0.8676
Baby’s weight
3546 (557)
3336 (333)
0.2906
3230 (407)
3498 (499)
0.3821
HbF (%)
88.5 (5.6)
91.5 (6.0)
0.2891
· (·)
90.1 (5.8)
·
Cord blood : CPD
1.71 (0.66)
2.01 (0.96)
0.3310
1.73 (0.70)
1.85 (0.82)
0.8113
ATP (μmol/g Hb)
2.87 (0.58)
2.68 (0.83)
0.4811
2.68 (0.46)
2.80 (0.72)
0.7936
Hemolysis (%)
0.12 (0.05)
0.12 (0.05)
0.7987
0.11 (0.02)
0.12 (0.05)
0.6607
MetHb (%)
4.54 (1.62)
4.67 (2.07)
0.8531
4.46 (1.68)
4.61 (1.84)
0.8941
2,3-DPG (mmol/L)
0.11 (0.15)
0.11 (0.26)
0.9933
0.05 (0.03)
0.12 (0.21)
0.5923
Deformability
0.445 (0.029)
0.447 (0.032)
0.8972
0.437 (0.014)
0.447 (0.031)
0.5834
Rigidity
1.41 (0.21)
1.56 (0.43)
0.2025
1.27 (0.23)
1.49 (0.33)
0.2747
Microparticle events (%)
0.3 (0.2)
0.2 (0.1)
0.2483
0.9 (0.1)
0.3 (0.2)
0.8861
PS-positive RBCs (%)
5.4 (1.0)
5.1(0.7)
0.3842
4.7 (1.4)
5.3 (0.8)
0.2113
CD47-positive RBCs (%)
99.9 (0.1)
99.8 (0.2)
0.0706
99.9 (0.1)
99.9 (0.1)
0.4089
PS-positive microparticles (%)
61.2 (21.8)
61.5 (11.2)
0.9770
57.2 (2.4)
61.9 (19.3)
0.6848
CD47-positive microparticles (%)
87.6 (30.1)
89.2 (10.2)
0.8674
76.3 (16.6)
89.8 (24.8)
0.3736
MFI of PS on RBCs
47 (7)
44(13)
0.3924
51 (10)
45 (10)
0.2715
MFI of CD47 on RBCs
275 (73)
271 (99)
0.8983
299 (114)
271 (82)
0.5814
MFI of PS on microparticles
324 (62)
324 (46)
0.9946
366 (74)
319 (52)
0.1730
MFI of CD47 on microparticles
837 (434)
694 (258)
0.3209
899 (336)
761 (378)
0.5528
The numbers in the table are absolute values for each parameter (standard deviation in brackets), *<0.05 (Student’s -test). HbF—fetal hemoglobin, CPD—citrate-phosphate-dextrose (anticoagulant), ATP—adenosine triphosphate, MetHb—methemoglobin, 2,3-DPG—2,3-diphosphoglycerate, PS—phosphatidylserine, and MFI—mean fluorescence intensity.
