Table of Contents
Journal of Blood Transfusion
Volume 2014, Article ID 204301, 4 pages
http://dx.doi.org/10.1155/2014/204301
Research Article

Testing for Partial RhD with a D-Screen Diagast Kit in Moroccan Blood Donors with Weak D Expression

1Centre National de Transfusion Sanguine, rue Lamfadel Cherkaoui, Madinat Al Irfane, P.O. Box 180, 10000 Rabat, Morocco
2Departement de Biologie, Faculté des Sciences, University MDV Rabat Agdal, 1014 Rabat, Morocco
3Centre Regional de Transfusion Sanguine, rue Lamfadel Cherkaoui, Madinat Al Irfane, P.O. Box 180, 10000 Rabat, Morocco

Received 5 July 2014; Revised 19 September 2014; Accepted 26 September 2014; Published 28 October 2014

Academic Editor: Maria Rios

Copyright © 2014 Z. Kabiri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to search for the partial D phenotype in Moroccan blood donors with weak D expression. The study included 32 samples with weak D phenotype, and partial D category red blood cells were detected with the D-Screen Diagast kit, which consists in 9 monoclonal anti-D antibodies specific for the most common categories of partial D. Among the 32 samples studied, we identified 13 specific reactions to a partial D antigen (3 DVI, 2 DVa, 2 D, and 6 DVII), with 8 reactions suggesting a weak D and 11 reactions providing no formal argument in favor of a partial D antigen. This work can be used to validate the performance of the anti-D reagent and to improve the safety of transfusion of red blood cells from donors expressing the partial D antigen by integrating the finding into the recipient file with a recommendation concerning the appropriate care.