Quantification of Cell-Free DNA in Red Blood Cell Units in Different Whole Blood Processing Methods

Table 2

The Interaction between storage duration and processing method on differences in cfDNA.

Assay	Red cell storage duration	Method of whole blood processing		value
		Whole blood filtration (WBF)	Red cell filtration (RCF)
cfDNA by PicoGreen (mean ng/mL ± SD)	Fresh (≤14 days)	1.16 ± 1.14; ()	0.37 ± 0.77; ()	0.0093
	Older (>14 days)	1.04 ± 0.78; ()	0.68 ± 0.74; ()	0.33
cfDNA by spectrophotometry (mean µg/mL ± SD)	Fresh (≤14 days)	4.15 ± 2.47; ()	3.63 ± 1.25; ()	0.67
	Older (>14 days)	3.27 ± 1.65; ()	2.75 ± 1.15; ()	0.57