Table of Contents
Journal of Coatings
Volume 2014, Article ID 256168, 8 pages
http://dx.doi.org/10.1155/2014/256168
Research Article

Simultaneous Bacillus anthracis Spores Detection via Aminated-Poly(vinyl chloride) Coated Piezoelectric Crystal Immunosensor

1Department of Medical Genetics, Gulhane Military Medical Academy, 06018 Ankara, Turkey
2Department of Chemistry, Science Faculty, Ankara University, 06100 Ankara, Turkey
3Department of Medical Microbiology, Gulhane Military Medical Academy, 06018 Ankara, Turkey

Received 30 May 2014; Accepted 28 July 2014; Published 19 August 2014

Academic Editor: Chao Zhang

Copyright © 2014 Ali Oztuna et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bacillus anthracis spores are a potential threat to countries in the context of biodefense. We have already seen the destructiveness of the anthrax attacks in the recent past. This study presents an aminated-poly(vinyl chloride) (PVC-NH2) coated quartz crystal microbalance (QCM) immunosensor for simultaneous rapid detection of B. anthracis spores. PVC-NH2, synthesized in the laboratory, was used as an adhesive layer for monoclonal antibody immobilization on gold quartz crystal. The prepared QCM sensor was tested using a pathogen field strain of B. anthracis (GenBank number: GQ375871.1) under static addition and flow through procedures with different spore concentrations. Fourier transform infrared spectroscopy (FTIR-ATR) and scanning electron microscopy (SEM) were performed to characterize the surface of the sensor during the modification. Furthermore, a series of SEM micrographs were taken in order to investigate surface morphology and show the presence of the B. anthracis spores on the surface. It is concluded that B. anthracis spores can be accomplished by using amine functionalized polymer coated QCM sensors without requiring complicated immobilization procedures or expensive preliminary preparations.