Highly Effective Gene Transfection In Vivo by Alkylated Polyethylenimine

Table 1

Biodistribution of ¹²⁵I-labeled gWiz Luc plasmid delivered with linear PEI and its N-alkylated derivatives. Values are the percentages of injected dose still remaining in select tissues at 24 h.

Polycation	Spleen	Kidney	Liver	Lung	Heart	Blood
unmodified PEI	0.03	0.11	0.49	0.01	0.00	0.02
N-methyl-PEI	0.02	0.08	0.30	0.01	0.00	0.03
N-ethyl-PEI	0.02	0.12	0.30	0.02	0.01	0.06
N-propyl-PEI	0.02	0.11	0.30	0.01	0.00	0.03
N-butyl-PEI	0.02	0.09	0.29	0.01	0.00	0.03
N-octyl-PEI	0.03	0.05	0.27	0.01	0.00	0.01