The Use of Radioactive Marker as a Tool to Evaluate the Drug Release in Plasma and Particle Biodistribution of Block Copolymer Nanoparticles
Figure 1
Column chromatography of nanoparticles. (a) Plasma from mice injected i.v. with 3[H] CHE-labelled Propac 7/POPC/PS 1 : 1 : 2 nanoparticles fractionated on a sepharose 4B column in HEPES buffered saline, pH 7.4. Column aliquots of were assayed for protein and 3[H]. The concentration of protein and 3[H] CHE for each fraction are plotted. (b) Plasma from mice injected i.v. with 3[H] CHE-labelled DSPE-2K nanoparticles were incubated either in water at room temperature or in mouse plasma at room temperature for one hour then fractionated on a sepharose 4B column. Column aliquots of were assayed for protein and 3[H]. The concentration of protein and 3[H] CHE for each fraction are plotted.
