Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR: Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery

Figure 2

Tethered planar bilayer formation on DMPE functionalized CM5 chip. A BIAcore CM5 chip was coated with DMPE as described in materials and methods. The functionalized chip was treated with DSPC/DSPE-DTPA containing vesicles at a 5 μL/min flow rate. The sensorgram shows the spreading of liposomes to a level of 1100 RU and the formation of a stable planar bilayer.