In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes

Figure 2

Effects of CyDs on leakage of calcein from calcein-encapsulated AF-liposomes in isotonic Tris-HCl-buffered saline (10 mM, pH 7.5) at 25°C. Calcein-encapsulated AF-liposomes were incubated with various CyDs for 30 min, and the concentrations of calcein released from AF-liposomes were determined using a fluorospectrophotometer. Each point represents the mean  ±  SEM of 3 experiments.