In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes
Figure 6
Effects of competitors on gene transfer activity of pDNA/γ-CyD/N-liposomes and AF-liposomes in HepG2 cells. pDNA was pRL-CMV. The charge ratio of AF-liposomes/pDNA was 1.6. γ-CyD was added to AF-liposomes suspension before freeze-drying. The concentration of γ-CyD was 1 μM/μM lipids. Cells were incubated with pDNA/γ-CyDs/AF-liposomes for 3 h in FCS-free medium. After washing twice, the cells were incubated for 21 h in culture medium supplemented with 10% FCS. The luciferase activity in cell lysates was determined using a luminometer. The concentrations of AF and BSA were 5 mg/mL. Each value represents the mean ± SEM of 3–7 experiments. * versus control.
