In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes
Figure 7
Gene transfer activity of pDNA/CyDs/AF-liposomes in (a) A549 cells and (b) NIH3T3 cells. pDNA was pRL-CMV. The charge ratio of AF-liposomes/pDNA was 1.6. CyDs were added to AF-liposomes suspension before freeze-drying. The concentrations of γ-CyDs were 1 μM/μM lipids. Cells were incubated with pDNA/γ-CyDs/AF-liposomes for 3 h in FCS-free medium. After washing twice, the cells were incubated for 21 h in culture medium supplemented with 10% FCS. The luciferase activity in cell lysates was determined using a luminometer. Each value represents the mean ± SEM of 3–7 experiments. * versus without CyD.
