In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes
Figure 8
Cytotoxicity of pDNA/AF-liposomes or pDNA/γ-CyDs/AF-liposomes with various charge ratios in (a) HepG2 and (b) NIH3T3 cells. The concentrations of γ-CyDs were 1 μM/μM lipids. Cells were incubated with pDNA/γ-CyDs/AF-liposomes for 3 h in FCS-free medium. Cell viability was assayed by the WST-1 method. Each point represents the mean ± SEM of 3 experiments.