Review Article
Liposome Technology for Industrial Purposes
Table 2
Methods of liposome preparation and the resulting product. Partly from Lasic and Barenholz [
39].
| Method | Vesicles |
| Mechanical methods | | Vortex or hand shaking of phospholipid dispersions | MLV | Extrusion through polycarbonate filters at low or medium pressure | OLV, LUV | Extrusion through a French press cell “Microfluidizer” technique | Mainly SUV | High-pressure homogenization | Mainly SUV | Ultrasonic irritation | SUV of minimal size | Bubbling of gas | BSV |
| Methods based on replacement of organic solvent(s) by aqueous media | Removal of organic solvent(s) | MLV, OLV, SUV | Use of water-immiscible solvents: ether and petroleum | MLV, OLV, LUV | Ethanol injection method | LUV | Ether infusion (solvent vaporization) | LUV, OLV, MLV | Reverse-phase evaporation | |
| Methods based on detergent removal | | Gel exclusion chromatography | SUV | “Slow” dialysis | LUV, OLV, MLV | Fast dilution | LUV, OLV | Other related techniques | MLV, OLV, LUV, SUV |
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